Author(s)
Ronald J. Schroeder MD
Juntao Luo
Brian Nicholas MD
Affiliation(s)
SUNY Upstate
Abstract:
Educational Objective: At the conclusion of this presentation, the participants should be able to discuss the pharmacokinetics of sodium thiosulfate in perilymph and its potential application as a localized therapy to prevent cisplatin induced ototoxicity. Objectives: To study the pharmacokinetics of sodium thiosulfate in the inner ear perilymph of guinea pigs using high pressure liquid chromatography. Study Design: Basic science laboratory research. Methods: Twenty guinea pig ears were split into two groups and administered sodium thiosulfate to the middle ear at either a concentration of 250 mg/mL or 50 mg/mL for 30 minutes. Perilymph samples were then obtained serially through the round window over 6 hours. Sodium thiosulfate concentrations were obtained using high pressure liquid chromatography. Results: The 250 mg/mL group had an initial perilymph concentration of 7.27 mg/mL (±0.83) and decreased to 0.94 mg/mL (±0.03) over 6 hours. The 50 mg/mL group had an initial concentration of 1.63 mg/mL (±0.17) and was undetectable after 1 hour. Conclusions: Sodium thiosulfate was shown to enter the inner ear perilymph via round window perfusion. This has a potential application as a localized therapy in the prevention of cisplatin induced ototoxicity.